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Expression of CD1 in the mouse thymus.
A Bradbury1, F Calabi, C Milstein
1MRC Laboratory of Molecular Biology, Cambridge.
European Journal of Immunology
|August 1, 1990
Summary
Researchers studied mouse CD1D gene expression in the thymus. They found CD1D mRNA predominantly in the cortex, with most of it unspliced, yet the corresponding protein product was detected.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- CD1 antigens are differentiation markers found in the human thymus.
- Five human CD1 genes (CD1A-E) exist, with CD1a-c identified by antibodies.
- Mouse CD1 genes include a homologue to human CD1D.
Purpose of the Study:
- To investigate the expression of mouse CD1D mRNA in the thymus.
- To determine the localization and splicing status of mouse CD1D mRNA.
- To confirm the presence of the CD1D protein product in mice.
Main Methods:
- Analysis of mouse thymus for CD1D mRNA expression.
- Assessment of mRNA splicing status.
- Detection of CD1D protein using a polyclonal antiserum against a bacterial fusion protein.
Main Results:
- Mouse CD1D mRNA expression is predominantly found in the thymic cortex.
- A significant portion of mouse CD1D mRNA in the thymus exists in an unspliced form.
- The expected CD1D protein product was identified despite the unspliced mRNA.
Conclusions:
- Mouse CD1D expression patterns in the thymus share similarities with human CD1 antigens.
- The presence of unspliced CD1D mRNA suggests complex post-transcriptional regulation.
- The study confirms the existence of the CD1D protein in mice, consistent with its homologue to human CD1D.