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Updated: Jul 19, 2026

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014
[Comparison between rapid detection method of enzyme substrate technique and multiple-tube fermentation technique in
Zong-ke Sun1, Rong Wu, Pei Ding
1Institute for Environment Hygiene and Health Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Objective:
To compare between rapid detection method of enzyme substrate technique and multiple-tube fermentation technique in water coliform bacteria detection.
Methods:
Using inoculated and real water samples to compare the equivalence and false positive rate between two methods.
Results:
Results demonstrate that enzyme substrate technique shows equivalence with multiple-tube fermentation technique (P = 0.059), false positive rate between the two methods has no statistical difference.
Conclusion:
It is suggested that enzyme substrate technique can be used as a standard method for water microbiological safety evaluation.

