Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Agrobacterium virulence gene induction.

Stanton B Gelvin1

  • 1Department of Biological Sciences, Purdue University, West Lafayette, IN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2006
PubMed
Summary

Agrobacterium transformation relies on specific phenolic compounds to activate virulence genes. Adding these compounds enhances Agrobacterium-mediated genetic transformation in species lacking sufficient natural inducers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Arabidopsis DNA repair mutants can integrate Agrobacterium T-DNA into the plant genome.

The New phytologist·2026
Same author

Myosin VIII and XI isoforms interact with the Agrobacterium VirE2 protein and facilitate its transport from the plasma membrane to the perinuclear region during plant transformation.

Plant communications·2025
Same author

Transformation and regeneration of DNA polymerase Θ mutant rice plants.

Plant direct·2023
Same author

Characterization of T-Circles and Their Formation Reveal Similarities to <i>Agrobacterium</i> T-DNA Integration Patterns.

Frontiers in plant science·2022
Same author

Plant DNA Repair and <i>Agrobacterium</i> T-DNA Integration.

International journal of molecular sciences·2021
Same author

<i>Agrobacterium</i> VirE2 Protein Modulates Plant Gene Expression and Mediates Transformation From Its Location Outside the Nucleus.

Frontiers in plant science·2021

Area of Science:

  • Microbiology
  • Molecular Biology
  • Plant Biotechnology

Background:

  • Agrobacterium-mediated transformation is a key tool in plant biotechnology.
  • The process is regulated by the Virulence (Vir) two-component system, involving VirA and VirG proteins.
  • This system senses phenolic compounds from wounded plant tissues.

Purpose of the Study:

  • To discuss conditions for efficient induction of Agrobacterium virulence genes.
  • To address limitations in transforming plant and nonplant species due to insufficient natural phenolic compounds.

Main Methods:

  • The study focuses on the genetic control of Agrobacterium virulence.
  • It examines the role of phenolic compounds and sugars in activating vir genes.
  • Conditions for adding phenolic inducers are discussed.

Main Results:

  • Phenolic compounds, with specific sugars, activate vir genes, leading to T-DNA transfer.
  • Many species require external addition of phenolic compounds for efficient Agrobacterium transformation.
  • Optimizing inducer addition is crucial for successful transformation.

Conclusions:

  • Efficient Agrobacterium-mediated genetic transformation depends on controlled induction of virulence genes.
  • Supplementing with phenolic compounds is necessary for transforming species with low natural induction.
  • Understanding these induction conditions is vital for advancing plant biotechnology.

Related Experiment Videos