Related Experiment Video
Updated: Jul 19, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
Selective product amplification of thymine photodimer by recognition-directed supramolecular assistance
W G Skene1, Volker Berl, Hélène Risler
1Laboratoire de Chimie Supramoléculaire, ISIS-Université Louis Pasteur, 8, allée Gaspard Monge, BP 70028, 67083, Strasbourg cedex, France.
Abstract:
Two symmetric ditopic supramolecular templates (1 and 2) each presenting two hydrogen bonding recognition subunits were synthesized. Each such subunit comprises the same donor and acceptor pattern, capable of binding a substrate molecule with complementary hydrogen bonding groups to form a supramolecular complex. Substrate molecules, such as thymine or uracil derivatives, yield 2 : 1 complexes with the acceptors involving two hydrogen bonds to each subunit with ideal orientation for subsequent [2 + 2] dimerization upon photoirradiation. Selective syn photoproduct formation and concomitant suppression of the trans isomer are favored by orientation of the two guest nucleobases within the template cleft. Complementary donor and acceptor hydrogen bonding induced positioning of the two substrates and steric hindrance within the template clefts are responsible for the selective product formation.
Related Concept Videos
Translesion DNA Polymerases
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Proofreading
Proofreading
Errors During Replication are Corrected by the DNA Polymerase Enzyme
Cycloaddition Reactions: MO Requirements for Photochemical Activation
Spontaneous and Induced Mutations
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...

