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Updated: Jul 19, 2026

High-Resolution Respirometry to Assess Mitochondrial Function in Human Spermatozoa
Published on: June 23, 2023
The functionality of mitochondria differentiates human spermatozoa with high and low fertilizing capability
Frédérique Gallon1, Carole Marchetti, Nathalie Jouy
1INSERM U814, Faculté de Médecine 1, Lille, France.
Abstract:
By using flow-cytometric sorting, we compared some of the major factors related to fertility in sperm subpopulations with high and low mitochondrial membrane potential (DeltaPsi(m)). Results demonstrate that DeltaPsi(m)(high) spermatozoa represents a subpopulation of sperm with high fertility performance because they have normal morphology, high motility values, and calcium ionophore-induced acrosome reaction, suggesting the importance of mitochondrial functionality for fertilizing capacity of human spermatozoa.
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