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Related Experiment Video

Updated: Jul 19, 2026

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
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Effector-triggered immunity by the plant pathogen Phytophthora.

Dinah Qutob1, Jennifer Tedman-Jones, Mark Gijzen

  • 1Agriculture and Agri-Food Canada, 1391 Sandford Street, London, Ontario N5V 4T3, Canada. qutobd@agr.gc.ca

Trends in Microbiology
|September 26, 2006
PubMed
Summary

A novel avirulence gene locus, Avr3b-Avr10-Avr11, was identified in potato late blight pathogen Phytophthora infestans. This locus, encoding a WD40 protein, is recognized by multiple potato resistance genes.

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Area of Science:

  • Plant Pathology
  • Genetics
  • Molecular Biology

Background:

  • Potato late blight, caused by Phytophthora infestans, is a devastating disease.
  • Understanding pathogen avirulence mechanisms is crucial for developing durable resistance in crops.

Purpose of the Study:

  • To identify and characterize novel avirulence (Avr) genes in Phytophthora infestans.
  • To investigate the genetic basis of pathogen recognition by potato resistance (R) genes.

Main Methods:

  • Genetic mapping and sequencing to identify the avirulence locus.
  • Bioinformatic analysis of the encoded protein structure and function.
  • Comparative analysis of the locus in different pathogen strains.

Main Results:

  • Discovery of the Avr3b-Avr10-Avr11 locus, distinct from previously identified Avr loci.
  • The locus encodes a large protein with a WD40 domain, showing similarity to transcription factors.
  • Gene amplification leading to truncated copies is characteristic of avirulent P. infestans strains.

Conclusions:

  • The Avr3b-Avr10-Avr11 locus represents a new target for potato R genes.
  • Understanding this locus provides insights into pathogen evolution and host-pathogen interactions.
  • This discovery aids in breeding potato varieties with enhanced resistance to late blight.