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In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Published on: July 8, 2020
Changes of maternal transcripts in oocytes from persistent follicles in cattle
Brandon M Lingenfelter1, Robert A Dailey, E Keith Inskeep
1Division of Animal and Veterinary Sciences, West Virginia University, Morgantown, West Virginia 26506-6108, USA.
Molecular Reproduction and Development
|September 26, 2006
Summary
Prolonged follicle dominance in cows alters oocyte gene expression, potentially impacting early embryo development. Persistent follicles showed lower MSY2, PARN, and YY1 mRNA, but higher PAP and eIF-4E transcripts.
Area of Science:
- Reproductive biology
- Molecular genetics
- Embryology
Background:
- Prolonged follicle dominance is linked to increased early embryonic loss in cattle.
- Understanding molecular changes in oocytes from persistent follicles is crucial for improving reproductive outcomes.
Purpose of the Study:
- To investigate if follicle persistence alters mRNA expression of key genes within the oocyte.
- To identify specific genes affected by prolonged follicle dominance that may influence early embryogenesis.
Main Methods:
- Cows were assigned to groups with growing or persistent follicles at specific estrous cycle stages.
- Hormonal treatments (prostaglandin F2alpha and progesterone) were administered to manipulate follicle dynamics.
- Oocytes were collected from ovaries at defined time points, and quantitative real-time PCR was used to measure mRNA levels of 10 selected genes.
Main Results:
- Oocytes from persistent follicles exhibited significantly lower mRNA abundances for MSY2, PARN, and YY1 compared to those from growing follicles.
- Conversely, PAP and eIF-4E transcript levels were significantly higher in oocytes from persistent follicles.
- These findings indicate altered gene expression related to transcription and translation regulation in oocytes from persistent follicles.
Conclusions:
- Follicle persistence in cows leads to significant changes in oocyte mRNA expression profiles.
- Altered gene expression, particularly in genes regulating transcription and protein translation, may compromise early embryonic development in cows ovulating persistent follicles.
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