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Construction and characterization of a novel maltose inducible expression vector in Bacillus subtilis
Yang Ming-Ming1, Zhang Wei-Wei, Zhang Xi-Feng
1Institute of Biochemical Engineering, College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, P.R. China.
Biotechnology Letters
|September 27, 2006
Abstract:
A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two genes, bioA and vgb. This thus provided a potential expression system for cloned genes in B. subtilis.
