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Updated: Jul 19, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
Published on: May 18, 2011
Fluorescence-suppressed Raman technique for quantitative analysis of protein solution using a micro-Raman probe, the
Yusuke Oshima1, Yuichi Komachi, Chie Furihata
1Tashiro Probing Technology Laboratory, Riken (The Institute of Physical and Chemical Research), Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Abstract:
A practical Raman analyzing technique with suppression of the strong fluorescent background in order to obtain quantitative information is proposed in the present study. The technique is based on the shifted excitation method and partial least squares regression (PLSR) analysis. The Raman system consists of a single Raman spectrometer, a background-free electrically tunable Ti:Sapphire laser (BF-ETL), and a micro-Raman probe (MRP). The system allows one to obtain reliable shifted excitation Raman spectra with a simple operation. The PLSR analysis successfully provides quantitative information from the obtained spectra with the suppression of random noise including photon shot noise. The present study demonstrates that the technique is effective for extracting quantitative information concealed behind a fluorescent background that is more than 200 times stronger than the Raman signal.
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