Related Experiment Video
Updated: Jul 19, 2026

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
[Effect of preparation technique on in vitro release mechanism of huperzine A microspheres]
Xu-dong Fu1, Yong-liang Gao, Qi-neng Ping
1Institute of Pharmacology and Toxicology, the Academy of Military Medical Sciences, Beijing 100850, China.
Aim:
To investigate the effect of preparation technique on in vitro release mechanism of huperzine A-PLGA microspheres.
Methods:
Huperzine A-PLGA microspheres were prepared by two kinds of O/O emulsion solvent evaporation method (method A and B). In vitro release mechanism was explained by release profile, degradation rate and swelling rate of microspheres in vitro. The microspheres morphology and drug distribution within microspheres were observed in order to explain further the drug release mechanism.
Results:
The encapsulation efficiency of huperzine A microspheres prepared by method A and B was 47.60% and 83.50% respectively. Microspheres prepared by method A could sustain release for 35 days with nearly no initial burst release. The release profile fitted well to zero order equation and drug release mainly through degradation and diffusion mechanism. Huperzine A microspheres prepared by method B could sustain release for 21 days with some evidence of initial burst release. The release profile fitted well to the Higuchi equation and drug release was mainly through diffusion mechanism.
Conclusion:
Huperzine A microspheres prepared by method A had more desirable release profile.
More Related Videos
07:24Preparation of Cross-Linked Sodium Alginate Microspheres with Different Metal Ions Using the Microfluidic Electrospray Technology
Published on: June 7, 2024
07:32Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles
Published on: August 28, 2015
Related Concept Videos
Factors Influencing Drug Absorption: Pharmaceutical Parameters
Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry
Factors Affecting Dissolution: Particle Size and Effective Surface Area
In Vitro Drug Dissolution: Alternative Methods