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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Absolute quantitation of microRNAs with a PCR-based assay

David D Duncan1, Mark Eshoo, Christine Esau

  • 1Isis Pharmaceuticals, Carlsbad, CA 92008, USA. d_duncan@att.net

Analytical Biochemistry
|September 30, 2006
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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