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Updated: Jul 19, 2026

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
DNA sequence-based detection of group B Streptococcus directly from heart valve tissue in a patient with
Kelly Guggisberg1, Robin Parker, David Megran
1Department of Pathology and Laboratory Medicine, Calgary, Alberta, Canada.
Abstract:
A human case of culture-negative bacterial endocarditis is presented where conventional methods failed to determine a microbial aetiology. DNA sequencing performed directly on autopsy heart valve tissue revealed Streptococcus agalactiae (group B streptococcus). To our knowledge, this is the first report of DNA sequence-based detection of this organism directly from a heart valve.
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