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Updated: Jul 19, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Published on: September 25, 2018
Insertion of two pyrene moieties into oligodeoxyribonucleotides for the efficient detection of deletion polymorphisms
Hiromu Kashida1, Hiroyuki Asanuma, Makoto Komiyama
1Research Center for Advanced Science and Technology, University of Tokyo, Komaba, Meguro-ku, Tokyo, 153-8904, Japan.
Abstract:
For the detection of deletion polymorphisms, two pyrene moieties are tethered to an oligodeoxyribonucleotide (ODN) on both sides of the intervening base; one- and two-base deletions can be selectively detected by the strength of the excimer emission.
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