Related Experiment Video
Updated: Jul 19, 2026
Remote Neuronal Activation Coupled with Automated Blood Sampling to Induce and Measure Circulating Luteinizing Hormone in Mice
Published on: August 25, 2023
Urinary beta-luteinizing hormone and beta-follicle stimulating hormone immunoenzymometric assays for population
Eleanor Brindle1, Rebecca C Miller, Jane B Shofer
1Center for Studies in Demography and Ecology, Box 353412, University of Washington, Seattle, Washington 98195, USA. ebrindle@u.washington.edu <ebrindle@u.washington.edu>
Objective:
We developed assays for measurement of urinary betaLH and betaFSH under collection and storage conditions typical of non-clinical research settings.
Design And Methods:
IEMAs for free betaLH and total betaFSH were validated by standard methods. Stability of urinary betaLH and betaFSH was tested across freeze-thaws and stored long term at 4 degrees C or -20 degrees C, or short term at room temperature, and with heating to dissociate the subunits.
Results:
The IEMAs exhibited acceptable parallelism, specificity, recovery (averaging 100% for betaLH, 97% for betaFSH), imprecision (maximum within-run and between run CVs, respectively, 4.8% and 25.7% for betaLH, 5.6% and 17.0% for betaFSH), and minimum detectable dose (2.5 pmol/L for betaLH, 6.8 pmol/L for betaFSH). Urine and serum measures were highly correlated (r=0.95 for LH, 0.86 for FSH). There was no consistent decline with any storage type. Dissociation of subunits by heating was needed for betaLH, but not betaFSH.
Conclusion:
These IEMAs measure free betaLH and total betaFSH, overcoming inter-individual variability in, and collection and storage effects on, subunit dissociation, without the need for urine preservatives.
