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Updated: Jul 19, 2026

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
Published on: February 8, 2015
Nonblinking and long-lasting single-molecule fluorescence imaging
Ivan Rasnik1, Sean A McKinney, Taekjip Ha
1Department of Physics, Emory University, Atlanta, Georgia 30322, USA. irasnik@physics.emory.edu
Abstract:
Photobleaching and blinking of fluorophores pose fundamental limitations on the information content of single-molecule fluorescence measurements. Photoinduced blinking of Cy5 has hampered many previous investigations using this popular fluorophore. Here we show that Trolox in combination with the enzymatic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and improves the signal linearity at high excitation rates, significantly extending the applicability of single-molecule fluorescence techniques.
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