The effects of salt-loading and dehydration on atrial natriuretic peptide (ANP) gene expression
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1Department of Biochemistry and Molecular Biology, Norman Bethune Medical University, Chang-Chun, Jilin Province, People's Republic of China.
Abstract:
1. Effects of sodium loading and dehydration on ANP gene expression were investigated in rats. 2. ANP-mRNA was determined using Northern blot and dot blot hybridization technique with alpha-32P-labeled r-preproANP-cDNA probe. Salt loading increased the ANP-mRNA content in atria. Correlation with ANP-mRNA content and the plasma sodium concentration was established. 3. Deprivation of water for 2 and 4 days increased ANP-mRNA 2.1- and 1.6-fold, respectively. 4. These results demonstrated that water-salt balance affects the ANP-gene expression.