Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expression of attachment proteins during cementogenesis.

M J Somerman1, B Shroff, W S Agraves

  • 1University of Maryland Dental School, Baltimore 21201.

Journal De Biologie Buccale
|September 1, 1990
PubMed
Summary

Researchers identified key cell attachment proteins in cementum crucial for root development. These proteins, including osteopontin, influence cell differentiation and attachment during tooth formation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dermal uptake of nicotine from air and clothing: Experimental verification.

Indoor air·2017
Same author

Advanced buffer materials for indoor air CO<sub>2</sub> control in commercial buildings.

Indoor air·2017
Same author

Spatial heterogeneity, population "regulation" and local extinction in simulated host-parasitoid interactions.

Oecologia·2017
Same author

Diagnostic accuracy of DXA compared to conventional spine radiographs for the detection of vertebral fractures in children.

European radiology·2016
Same author

Alignment and component position after patient-matched instrumentation versus conventional total knee arthroplasty.

Journal of orthopaedic surgery (Hong Kong)·2016
Same author

Measurements of dermal uptake of nicotine directly from air and clothing.

Indoor air·2016

Area of Science:

  • Biochemistry
  • Developmental Biology
  • Cell Biology

Background:

  • The extracellular environment is vital for cell differentiation during development.
  • Cell attachment proteins and receptors are potential regulators of this process.
  • Understanding cementum proteins is key to root formation.

Purpose of the Study:

  • Identify attachment proteins in mature cementum.
  • Determine the function of these proteins during root formation.
  • Investigate the role of specific proteins like osteopontin.

Main Methods:

  • Fractionation of cementum extracts to isolate proteins.
  • Immunofluorescent examination of developing mouse molar tissues.
  • In vitro cell attachment assays using osteopontin-coated dishes.

Related Experiment Videos

Main Results:

  • Bone sialoprotein (BSP) and other undetermined attachment proteins were found in cementum.
  • Osteopontin was detected in Hertwig's epithelial root sheath during root formation.
  • Dental papilla cells, capable of cementum formation, attached to osteopontin in vitro.

Conclusions:

  • Unique and known attachment proteins are present in mature cementum and during root development.
  • Osteopontin plays a role in cell attachment relevant to cementum formation.
  • Further research on protein localization is needed to fully elucidate their function in root development.