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The activation of TC10, a Rho small GTPase, contributes to v-Rel-mediated transformation

S Tong1, A S Liss, M You

  • 1Section of Molecular Genetics and Microbiology and the Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712-1095, USA.

Oncogene
|October 4, 2006
PubMed

Insights

The oncogenic transcription factor v-Rel increases TC10 Rho GTPase expression, promoting cell transformation. Elevated TC10 activity alone can induce in vitro transformation of primary cells.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Biology

Background:

  • v-Rel, an oncogenic transcription factor from the Rel/NF-kappaB family, induces cellular transformation.
  • Understanding genes regulated by v-Rel is crucial for deciphering its oncogenic mechanisms.

Purpose of the Study:

  • To identify genes with altered expression in v-Rel-transformed cells.
  • To investigate the role of TC10, a Rho GTPase, in v-Rel-mediated cell transformation.

Main Methods:

  • Differential display was used to identify differentially expressed genes.
  • Expression levels of TC10 were analyzed at RNA and protein levels.
  • Functional assays involved dominant-negative and gain-of-function TC10 mutants.

Main Results:

  • TC10 expression and active GTP-bound levels were significantly increased in v-Rel-transformed chicken embryonic fibroblasts (CEFs).
  • Dominant-negative TC10 reduced colony formation, while TC10 overexpression enhanced it.
  • Overexpression of TC10 alone promoted anchorage-independent growth and increased saturation density in CEFs.

Conclusions:

  • Elevated TC10 activity is a key contributor to v-Rel-mediated transformation of CEFs.
  • Rho factors, like TC10, can alone induce in vitro transformation of primary cells.

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