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Updated: Jul 19, 2026
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
Published on: November 10, 2016
[Application of p-aminothiophenol as a SERS reporter in immunoassay]
Yun Jiang1, Yan Cui, Ren-Ao Gu
1Department of Chemistry and Chemical Engineering, Suzhou University, Suzhou 215006, China.
The adsorption of p-aminothiophenol (PATP) molecule on the gold electrode was investigated. The result suggests that PATP was adsorbed perpendicularly onto the substrate. By immobilizing goat anti-mouse IgG on a glass slide modified by special organic molecules, the antibody/antigen/reporter-labeled immunogold nanoparticle sandwich assay was build for surface-enhanced Raman spectroscopy (SERS) measurements. The p-aminothiophenol (PATP) was used as SERS-reporter molecule. Thus the authors can identify the single type biospecific species by SERS signal of the reporter molecules which are coimmobilized with antibody molecules in gold colloids.
The adsorption of p-aminothiophenol (PATP) molecule on the gold electrode was investigated. The result suggests that PATP was adsorbed perpendicularly onto the substrate. By immobilizing goat anti-mouse IgG on a glass slide modified by special organic molecules, the antibody/antigen/reporter-labeled immunogold nanoparticle sandwich assay was build for surface-enhanced Raman spectroscopy (SERS) measurements. The p-aminothiophenol (PATP) was used as SERS-reporter molecule. Thus the authors can identify the single type biospecific species by SERS signal of the reporter molecules which are coimmobilized with antibody molecules in gold colloids.
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