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Updated: Jul 19, 2026

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
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Published on: September 18, 2013

LRRK2 expression in normal and pathologic human brain and in human cell lines.

Judith Miklossy1, Tetsuaki Arai, Jian-Ping Guo

  • 1Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, BC, Canada.

Journal of Neuropathology and Experimental Neurology
|October 6, 2006
PubMed
Summary

Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene are linked to Parkinson disease. LRRK2 protein is found in human brain cells and aggregates in various neurodegenerative disorders.

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06:09

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Area of Science:

  • Neuroscience
  • Genetics
  • Pathology

Background:

  • Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene are associated with autosomal-dominant late-onset Parkinson disease.
  • LRRK2 is implicated in the pathogenesis of Parkinson disease.

Purpose of the Study:

  • To investigate the expression and localization of LRRK2 protein in the human brain and its association with neurodegenerative diseases.

Main Methods:

  • Reverse transcriptase-polymerase chain reaction (RT-PCR) to detect LRRK2 mRNA.
  • Western blotting to detect LRRK2 protein expression.
  • Immunocytochemistry to determine LRRK2 protein localization in normal brain tissue and pathological inclusions.

Main Results:

  • LRRK2 mRNA and protein are expressed in various human tissues, including the brain, and in cultured glial and neuronal cells.
  • The LRRK2 antibody recognizes neurons, astrocytes, and microglia in normal brain tissue.
  • LRRK2 intensely labels Lewy bodies in Parkinson disease and other pathological inclusions in multiple neurodegenerative disorders, including Alzheimer disease, Huntington disease, and amyotrophic lateral sclerosis.

Conclusions:

  • LRRK2 is constitutively expressed in both neurons and glial cells in the human brain.
  • LRRK2 protein strongly associates with pathological inclusions across a spectrum of neurodegenerative disorders, suggesting a potential role in their pathogenesis.