A method for determining pyrene in mucus using synchronous fluorimetry with multiple standard additions.

Area of Science:

• Analytical Chemistry
• Environmental Science
• Biomedical Analysis

Background:

• Pyrene is a polycyclic aromatic hydrocarbon (PAH) found in various environmental and biological samples.
• Accurate determination of pyrene in complex matrices like mucus is crucial for health and environmental monitoring.
• Existing methods often require extensive sample pretreatment, increasing time and cost.

Purpose of the Study:

• To develop and validate a novel, direct method for pyrene determination in mucus.
• To compare the performance of the new method against a standard GC/MS technique.
• To assess the efficiency and cost-effectiveness of the proposed analytical approach.

Main Methods:

• Development of a synchronous fluorimetry with multiple standard addition (SFMSA) method.
• Direct analysis of pyrene in mucus samples without prior solvent extraction or pretreatment.
• Validation of SFMSA using gas chromatography-mass spectrometry (GC/MS) as a reference method.

Main Results:

• The SFMSA method achieved a low method detection limit (MDL) of 0.47 ng/ml for pyrene in mucus.
• Linearity was observed in the standard addition graph within the 0.05-50.00 ng/ml range (r(2) = 0.9989).
• SFMSA demonstrated good agreement with GC/MS, showing higher average recovery and lower MDL.

Conclusions:

• SFMSA provides a direct, rapid, and cost-effective alternative for pyrene determination in mucus.
• The method eliminates the need for laborious solvent extraction, making it more practical for routine analysis.
• SFMSA offers improved sensitivity and efficiency compared to conventional GC/MS methods for pyrene in mucus.