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Updated: Feb 17, 2026

Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
Published on: January 22, 2019
Cofactor-independent phosphoglycerate mutase is an essential gene in procyclic form Trypanosoma brucei
Appolinaire Djikeng1, Sylvine Raverdy, Jeremy Foster
1The Institute for Genomic Research (TIGR), 9712 Medical Center Drive, Rockville, MD 20850, USA. adjikeng@tigr.org
Abstract:
Glycolysis and gluconeogenesis are, in part, driven by the interconversion of 3- and 2-phosphoglycerate (3-PG and 2-PG) which is performed by phosphoglycerate mutases (PGAMs) which can be cofactor dependant (dPGAM) or cofactor independent (iPGAM). The African trypanosome, Trypanosoma brucei, possesses the iPGAM form which is thought to play an important role in glycolysis. Here, we report on the use of RNA interference to down-regulate the T. brucei iPGAM in procyclic form T. brucei and evaluation of the resulting phenotype. We first demonstrated biochemically that depletion of the steady state levels of iPGM mRNA correlates with a marked reduction of enzyme activity. We further show that iPGAM is required for cell growth in procyclic T. brucei.
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