In vitro evaluation of a closed-bowel technique for one-layer hand-sewn inverting end-to-end jejunojejunosotomy in
1Department of Animal Pathology, Faculty of Veterinary Medicine, University of Turin, Turin, Italy. marco.gandini@unito.it
Objective:
To report a technique for closed-bowel 1-layer inverting end-to-end jejunojejunal anastomosis in horses.
Study Design:
Experimental study.
Sample Population:
Fresh cadaveric jejunal segments from 12 horses.
Methods:
For each bowel segment a 1-layer closed and a 2-layer inverting end-to-end jejunojejunosotomy was created. Anastomosis construction time and anastomotic bursting pressure were measured and compared.
Results:
Closed-bowel anastomosis was significantly faster to create than a 2-layer technique. Luminal narrowing (<30%) was similar with both techniques and comparable with other inverting techniques. Bursting pressure was significantly higher for the 2-layer technique, although all anastomoses resisted pressures higher than those reported for other jejunojejunal anastomosis techniques.
Conclusions:
A 1-layer hand-sewn, closed, inverting jejunojejunosotomy using a modified Doyen clamp was easy and faster to perform, and resulted in functional characteristics similar to, a 2-layer hand-sewn inverting technique.
Clinical Relevance:
A closed, 1-layer inverting technique could be considered for equine jejunal anastomosis but requires in vivo evaluation before recommendation for clinical use.

