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Human neurochordins: identification of several P-epitope-bearing polypeptides and a study of their ontogenetic
A A Preobrazhensky1, A I Rodionova, Yaroshevich EYu
1A.N. Bach Institute of Biochemistry, USSR Academy of Sciences, Moscow.
Abstract:
Neurochordins are a family of immunologically related high-Mr neural tissue glycoproteins. After SDS-agarose electrophoresis of human neural tissue extracts, two main neurochordins (A1 and B2) as well as several minor ones (O, A2, A3, B1, B3, C1, C2, D) were visualized on immunoblots stained with monoclonal antibody At5. Neurochordin expression starts in human embryos before 6 weeks of gestation. General antigenic activity of neurochordins increases between 6 and 24 weeks of gestation while its level does not alter from the second half of gestation up to the age of 11-13 years. Neurochordins extracted from large hemispheres of brain, from cerebellum and spinal cord of a 24-week embryo display a similar pattern after electrophoresis. Partially different pattern of neurochordins was observed with a brain tumor.