Evaluation of renal functional parameters in different settings of isolated organ hemoperfusions

Isolated porcine kidneys are commonly used to study physiological and pathophysiological aspects of renal homeostasis but standardized evaluation procedures of renal function in this model do not exist so far. A double-logarithmical nomogram is established for filtration and reabsorption functions in isolated and hemoperfused porcine kidneys using different perfusion settings. Model validity was demonstrated by the levels of urine flow and sodium excretion showing expected alteration levels of lowering in the ADH-group and increasing in the furosemide-group of isolated kidneys. Creatinine-clearance values were in constant ranges within each specific perfusion group as indicated by the nomogram procedure. The present studies used a nomogram method to analyze the effects of different renal perfusion settings in a porcine model of kidney perfusion. The method may be of use to differentiate various kidney perfusion parameters both at the experimental and clinical levels.