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Updated: Jul 19, 2026

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities
Published on: May 27, 2015
Sensitive and rapid electrochemical bioassay of glycosidase activity
Tanin Tangkuaram1, Jared Q Gerlach, Yun Xiang
1The Biodesign Institute and Fulton School of Engineering, Arizona State University, Tempe, AZ 85287-5801, USA.
Abstract:
Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.
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