Related Experiment Video
Updated: Jul 19, 2026

Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans
Published on: October 5, 2020
Cloning and characterization of a novel small monomeric GTPase, RasL10B, with tumor suppressor potential
1State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai, PR China.
Abstract:
Ras proteins are members of the superfamily of small GTPase. A novel human Ras-like transcript, termed RasL10B, was isolated from human blood cell cDNA library. RasL10B gene contains four exons and three introns, which encodes a 203 amino acid protein with a molecular mass of about 23.2 kDa. RT-PCR analysis showed that RasL10B is expressed extensively in human tissues. Subcellular location analysis of GFP-RasL10B fusion protein revealed that RasL10B was distributed to the cytoplasm of COS7 cells. In addition, RasL10B was expressed in E. coli Rosette (DE3) and purified to a homogenicity by Ni-NTA affinity chromatography. Finally, the mRNA levels of RasL10B were down-regulated in all human breast cancer cell lines we tested. In summary, RasL10B is a new member of Ras superfamily with tumor suppressor potential.
Related Concept Videos
The Ras Gene
Ras is a superfamily...
Small GTPases - Ras and Rho
Three regulatory proteins control their activity:
The Ras Gene
Ras is a superfamily...
The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
Abnormal Proliferation
Rab Proteins
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...

