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![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
Published on: December 4, 2017
Electrochemical studies on horseradish peroxidase covalently coupled with redox dyes
B S B Salomi1, Chanchal K Mitra
1School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
Abstract:
The present study aims at investigating the use of redox dyes as non-diffusional electron mediators in hydrogen peroxide biosensors using horseradish peroxidase (HRP). We observe that the two redox dyes Safranine O and Neutral Red covalently bound to HRP, efficiently mediate electron transfer from the active site of the enzyme to the electrode surface. Dyes bound to the enzyme using a spacer arm diaminohexane further enhance the electron transfer. The enzyme electrodes show a linear response to the concentration of H2O2 up to 500 microM concentration and with a detection limit of around 50 microM. The dyes can be used as coupled mediators to develop a successful electro-optical biosensor.
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