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Updated: Jul 19, 2026
13:01
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice
Published on: August 12, 2011
Mammalian cells
Tzvi Tzfira1, Talya Kunik, Yedidya Gafni
1Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 13, 2006
Summary
Agrobacterium can genetically transform mammalian cells, expanding its host range beyond plants. This opens new avenues for studying plant-specific transformation factors using mammalian cell systems.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Agrobacterium is known for its ability to genetically transform numerous plant species.
- Its host range extends beyond plants to fungi and prokaryotes.
- Recent findings show Agrobacterium can transform mammalian cells, including HeLa cells.
Purpose of the Study:
- To present procedures for Agrobacterium-mediated genetic transformation of mammalian cells.
- To demonstrate the utility of mammalian cells for investigating plant-specific transformation factors.
- To explore the cellular components involved in the genetic transformation pathway.
Main Methods:
- Development of basic procedures for transfection of mammalian cells.
- Establishment of Agrobacterium-mediated genetic transformation protocols for mammalian cells.
- Utilizing mammalian cells as an experimental system to study transformation pathways.
Main Results:
- Successful genetic transformation of mammalian cells using Agrobacterium.
- Demonstration of mammalian cells as a viable system for studying Agrobacterium transformation.
- Identification of potential plant-specific factors involved in the transformation process.
Conclusions:
- Agrobacterium's host range is broader than previously thought, including mammalian cells.
- Mammalian cells offer a valuable model for dissecting the molecular mechanisms of Agrobacterium-mediated genetic transformation.
- This research facilitates the identification and characterization of key factors in plant genetic engineering.
