Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mushroom (Agaricus bisporus).

C Peter Romaine1, Carl Schlagnhaufer

  • 1Department of Plant Pathology, The Pennsylvania State University, University Park, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 13, 2006
PubMed
Summary
This summary is machine-generated.

A new genetic transformation method for Agaricus bisporus uses Agrobacterium tumefaciens and fruiting body tissue for efficient gene transfer. This breakthrough overcomes previous limitations, enabling high-frequency transformation in edible mushrooms.

Related Experiment Videos

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-distance translocation of protein during morphogenesis of the fruiting body in the filamentous fungus, Agaricus bisporus.

PloS one·2011
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Area of Science:

  • Mycology
  • Molecular Biology
  • Biotechnology

Background:

  • Genetic transformation is crucial for improving crop traits.
  • Traditional methods for transforming the edible mushroom Agaricus bisporus have faced challenges with efficiency.
  • Agrobacterium tumefaciens-mediated transformation is a powerful tool in plant and fungal genetics.

Purpose of the Study:

  • To develop an efficient and easy genetic transformation protocol for Agaricus bisporus.
  • To overcome the low transformation rates associated with previous methods targeting spores or mycelium.
  • To utilize the reproductive fruiting body for enhanced Agrobacterium-mediated gene transfer.

Main Methods:

  • Exploited the T-DNA transfer mechanism of Agrobacterium tumefaciens.
  • Utilized reproductive fruiting body explants, specifically the mushroom gill, as recipient tissue.
  • Incubated A. tumefaciens with acetosyringone, co-cultivated with gill explants, and selected transformants using hygromycin resistance.

Main Results:

  • Achieved high-frequency transformation, with over 95% of gill explants generating hygromycin-resistant colonies within 7-28 days.
  • Demonstrated successful gene integration, with approximately 75% of transformants showing a single copy of the resistance gene.
  • Established an effective method overcoming limitations of targeting fungal spores or vegetative mycelium.

Conclusions:

  • The developed method offers an easy and effective approach for genetic transformation of Agaricus bisporus.
  • This protocol significantly enhances transformation efficiency by using fruiting body tissue.
  • The findings pave the way for improved genetic manipulation and breeding of this important edible mushroom.