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Updated: Jul 19, 2026

Anchorage of Twisted Testis: A Rat Model of Ischemia-Reperfusion Injury of the Testis
Published on: November 14, 2025
Long-term influence of prepubertal testicular torsion on spermatogenesis
Jie Sun1, Guo-hua Liu, Hai-teng Zhao
1Department of Surgery, Xin Hua Hospital and Shanghai Children's Medical Center, Shanghai, PR China. mailtosun99@yahoo.com.cn
Purpose:
To study the influence of prepubertal unilateral testicular torsion on spermatogenesis postpubertally.
Methods:
Sixty prepubertal SD male rats were divided into 6 groups. In each group, animal suffered different courses of unilateral testicular torsion including sham operation, 2- and 6-hour-long torsion, and permanent torsion. Salvia miltiorrhiza was injected as a remedy to release the I/R injury in the 2- and 6-hour-long torted groups. Postpubertally, the percentage of DNA content of haploid cells in the testes was determined individually.
Results:
The percentage of haploid cells in the sham operation group was 76.5+/-1.9%. The number decreased in every other group (p<0.01). Comparing with the same course of torsion, Salvia miltiorrhiza injection could improve the percentage of haploid cells (p<0.01). In the permanent unilateral testicular torted group, the percentage was nearly zero (0.4+/-0.2%).
Conclusions:
Prepubertal unilateral testicular torsion induces decreased spermatogenesis postpubertally. The result takes place in the bilateral testes. Salvia miltiorrhiza, as an antioxidant remedy, could relieve the injury which manifests improved spermatogenesis.
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