Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lysozyme binding onto cat-anionic vesicles.

A Bonincontro1, E Spigone, M Ruiz Peña

  • 1CNISM-Department of Physics, University of Rome La Sapienza, P. le A. Moro 5, I-00185 Roma, Italy.

Journal of Colloid and Interface Science
|October 13, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

COVID-19 and kidney: role of SARS-CoV-2 infection in the induction of renal damage.

European review for medical and pharmacological sciences·2023
Same author

Kidney dysfunction is associated with adverse outcomes in internal medicine COVID-19 hospitalized patients.

European review for medical and pharmacological sciences·2023
Same author

Analysis of prognostic factors in COVID-19 hospitalized patients: an Italian single-center case-control study.

European review for medical and pharmacological sciences·2023
Same author

Relationship between plasma aldosterone levels and arterial stiffness parameters in hypertensive patients with subclinical vascular damage.

International journal of cardiology. Cardiovascular risk and prevention·2022
Same author

A case of chronic systemic capillary leak syndrome (SCLS) exacerbated during SARS-CoV2 infection.

European review for medical and pharmacological sciences·2021
Same author

Criticism of learning curve in laparoscopic adrenalectomy: a systematic review.

La Clinica terapeutica·2020

Cat-anionic vesicles formed from sodium dodecylsulfate and cetyltrimethylammonium bromide interact with lysozyme, creating lipo-plexes. Protein release occurs when excess sodium dodecylsulfate is neutralized, preserving lysozyme

Area of Science:

  • Colloid and Surface Science
  • Biophysical Chemistry
  • Materials Science

Background:

  • Formation of cat-anionic vesicles from mixed surfactants sodium dodecylsulfate (SDS) and cetyltrimethylammonium bromide (CTAB).
  • Electrostatic interactions between negatively charged vesicles and positively charged proteins like lysozyme.
  • Understanding protein-surfactant interactions is crucial for drug delivery and biomaterial applications.

Purpose of the Study:

  • To investigate the formation and properties of lipo-plexes formed between SDS/CTAB vesicles and lysozyme.
  • To characterize the binding, conformation, and release of lysozyme from these vesicles.
  • To determine the conditions necessary for protein release from the lipo-plexes.

Main Methods:

  • Preparation of cat-anionic vesicles using specific SDS/CTAB mole ratios.

Related Experiment Videos

  • Characterization of vesicle-protein interactions using dielectric relaxation, zeta-potential, and light scattering.
  • Assessment of lysozyme conformation using Circular Dichroism (CD) spectroscopy.
  • Investigation of protein release mechanisms by altering surfactant concentrations.

    • Main Results:

    • Formation of stable lipo-plexes through electrostatic interactions between SDS/CTAB vesicles and lysozyme.
    • Lysozyme retains its native conformation upon adsorption to the vesicles.
    • Vesicle clustering and lipo-plex flocculation are induced by adsorbed lysozyme.
    • Protein release in native form is achieved by neutralizing excess SDS with CTAB, leading to lipo-plex break-up.

    Conclusions:

    • SDS/CTAB vesicles can effectively bind and stabilize lysozyme without compromising its native structure.
    • The stoichiometry of charge neutralization dictates the formation and stability of lipo-plexes.
    • Controlled release of native lysozyme can be triggered by specific changes in the surfactant composition, offering potential for controlled delivery systems.