Related Experiment Video

Updated: Jul 19, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

Physical and functional interaction of the p14ARF tumor suppressor with ribosomes

Helen Rizos1, Heather A McKenzie, Ana Luisa Ayub

  • 1Westmead Institute for Cancer Research, University of Sydney at Westmead Millennium Institute, Westmead Hospital, Westmead, New South Wales 2145, Australia. helen_rizos@wmi.usyd.edu.au

The Journal of Biological Chemistry
|October 13, 2006
PubMed

Insights

The tumor suppressor p14ARF interacts with ribosomes, impacting protein translation. This suggests a new pathway for p14ARF in regulating cell growth, complementing its known p53 functions.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • p14ARF is a tumor suppressor frequently altered in human cancers.
  • Its role in the nucleolus and ribosome biogenesis is controversial.
  • p14ARF is known to regulate p53 and the protein B23.

Purpose of the Study:

  • To clarify the nucleolar functions of p14ARF.
  • To investigate the interaction of p14ARF with ribosomes.
  • To determine the effect of p14ARF on ribosome biogenesis and protein translation.

Main Methods:

  • Studying the interaction between p14ARF and ribosomes.
  • Analyzing the effect of p14ARF expression on polysome formation and protein translation in vivo.
  • Investigating the interaction of p14ARF with the endoribonuclease B23 within the nucleolar 60 S preribosomal particle.

Main Results:

  • p14ARF and B23 interact within the 60 S preribosomal particle.
  • This interaction does not require rRNA.
  • p14ARF expression inhibits polysome formation and protein translation in vivo, without significantly altering ribosome biogenesis.

Conclusions:

  • p14ARF has a ribosome-dependent pathway that regulates cell growth.
  • This pathway complements the known p53-dependent functions of p14ARF.
  • p14ARF's role in regulating protein translation provides new insights into its tumor suppressor activities.

More Related Videos

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
Termination of Translation01:44

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...