Laboratory diagnosis of respiratory infections

The laboratory diagnosis of infections of the respiratory tract is not an exact science, with many clinicians electing to empirically select antimicrobial therapy without the benefit of laboratory testing. With trained laboratory personnel and the proper selection of tests, accurate laboratory diagnosis is available. Progress is occurring most rapidly with molecular methods, such as polymerase chain reaction (PCR) testing. As molecular approaches are technically simplified and become less expensive, advances in the laboratory diagnosis of most respiratory tract infections caused by fastidious pathogens will occur. The diagnosis of non-fastidious bacteria, that require in-vitro antimicrobial testing, will continue to require conventional culture methods. New bronchoscopic methods, quantitative evaluation of cultures, and recognition of intracellular bacteria in stained smears do improve the usefulness of conventional culture and stain in the diagnosis of pneumonia.