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Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy
12:34

Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy

Published on: December 13, 2012

Fluorescence visualization of newly synthesized proteins in mammalian cells

Kimberly E Beatty1, Julie C Liu, Fang Xie

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA.

Angewandte Chemie (International Ed. in English)
|October 13, 2006
PubMed
Abstract

No abstract available in PubMed .

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...