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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Unlocking the hidden DNAzyme repertoire through aptamer-gated exonuclease signal amplification

Shusen Xiao1,2, Kai Niu1,2, Yangyang Chang1,2

  • 1Central Hospital of Dalian University of Technology, Dalian Medical-Engineering Interdisciplinary Rapid Diagnosis of Major Diseases Laboratory, Dalian University of Technology, Dalian, 116000 China. yuanhonglab@163.com.

Chemical Communications (Cambridge, England)
|September 7, 2026
PubMed

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Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...