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Updated: Jul 19, 2026
Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software
Published on: December 14, 2020
[Quantification for biofilm formation of pseudomonas aeruginosa isolates and their genotypic analysis]
Xi Cheng1, Wei-qing Yang, Lei Shi
1Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To probe the correlation between the ability of biofilm formation of Pseudomonas aeruginosa isolates and their genotypes.
Methods:
Forty-eight Pseudomonas aeruginosa isolates were tested for their biofilm formation with a modified microtiter test and were analyzed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The percent similarity between their genetic fingerprints and cluster analysis was performed and worked out using computer software.
Results:
Forty-eight Pseudomonas aeruginosa isolates demonstrated different abilities of biofilm formation in vitro. And the 48 isolates with different abilities of biofilm formation showed different genotypes in fingerprints by ERIC-PCR. Among the 48 isolates of Pseudomonas aeruginosa tested, five clusters (A, B, C, D, E), were identified at the 17% genetic similarity level. The isolates with strong ability of biofilm formation were in D cluster at the 42% genetic similarity level.
Conclusion:
Most of the 48 PA isolates tested formed strong biofilm, and their genotypes were of correlation.
