Related Experiment Video
Updated: Jul 19, 2026
Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea
Published on: December 23, 2022
[Determination of ginsenoside Rd and its metabolites in rat urine by LC-MS]
Liu Yang1, Shun-jun Xu, Xing Zeng
1Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine pathways, Guangzhou 510120, China.
Aim:
To study the metabolic pathways of ginsenoside Rd in rats.
Methods:
Urine samples were collected before and after 24 h of single oral administration of 150 mg and intravenous administration of 60 mg of ginsenoside Rd to six rats, separately. The samples were purified by SPE column and then were analyzed by liquid chromatography-ESI-mass spectrometry for putative metabolites.
Results:
Parent drug and its seven metabolites were identified in rat urine based on comparing total ion chromatograms of the blank with the metalolic urine as well as mass spectra. Its main metabolic pathways and possible structures are elucidated.
Conclusion:
Oxidation, combination and deglucosylation were found to be the major metabolic pathway of ginsenoside Rd in rats.
More Related Videos
05:59An In Vitro Dissolution Determination of Multi-Index Components in Tibetan Medicine Rhodiola Granules
Published on: November 4, 2022
08:41Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017