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[Harmine induces apoptosis in human SGC-7901 cells]
Zhen-ya Song1, Ji-ren Liu, Xin-liang Lu
1Department of Gastroenterology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.
Objective:
[corrected] To investigate the effect of apoptosis induced in human SGC-7901 cells by Harmine.
Methods:
The effect of Harmine on human SGC-7901 cell survival and apoptosis was determined by MTT assay, light microscopy and flow cytometry. Cell genomic DNA was detected by agarose electrophoresis.
Results:
The survival of human SGC-7901 cells decreased; Apoptotic cells were observed by fluorescent microscope; FCM analysis showed that the peak of apoptosis increased. Typical DNA Ladder were detected in DNA agarose gel electrophoresis.
Conclusion:
HM can induce apoptosis in human SGC-7901 cells.