Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A biologically relevant antioxidative action of PADMA28 in human in-vivo is questionable.

VASA. Zeitschrift fur Gefasskrankheiten·2005
Same author

Modification of low-density lipoprotein by different radioiodination methods.

Nuclear medicine and biology·2004
Same author

Troglitazone-binding to LDL and its glycated modifications: its role in cell-catalysed and Cu-mediated LDL-oxidation.

Life sciences·2003
Same author

Binding of [99mTc]chondroitin sulfate to scavenger receptors on human chondrocytes as compared to binding of oxidized [125I]LDL on human macrophages.

Journal of receptor and signal transduction research·2002
Same author

Antibodies against oxidized LDL in infants.

Clinical chemistry·2001
Same author

Prostaglandins and lipid modification.

Current pharmaceutical design·2001

Related Experiment Video

Updated: Jul 19, 2026

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method
09:08

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method

Published on: December 20, 2024

Modification of low-density lipoprotein during radiolabeling with 99mTc using three labeling methods.

G Sobal1, U Resch, F Tatzber

  • 1Department of Nuclear Medicine, Medical University of Vienna, Vienna, Austria. grazyna.sobal@meduniwien.ac.at

The Quarterly Journal of Nuclear Medicine and Molecular Imaging : Official Publication of the Italian Association of Nuclear Medicine (AIMN) [And] the International Association of Radiopharmacology (IAR), [And] Section of the Society Of
|October 18, 2006
PubMed
Summary

Radiolabeling low-density lipoprotein (LDL) with technetium-99m (99mTc) results in oxidative modification, making native-LDL imaging impossible. The ascorbic acid method offers some protection against LDL modification during labeling.

More Related Videos

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
11:34

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

Positron Emission Tomography Imaging of Cell Trafficking: A Method of Cell Radiolabeling
10:07

Positron Emission Tomography Imaging of Cell Trafficking: A Method of Cell Radiolabeling

Published on: October 27, 2023

Related Experiment Videos

Last Updated: Jul 19, 2026

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method
09:08

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method

Published on: December 20, 2024

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
11:34

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

Positron Emission Tomography Imaging of Cell Trafficking: A Method of Cell Radiolabeling
10:07

Positron Emission Tomography Imaging of Cell Trafficking: A Method of Cell Radiolabeling

Published on: October 27, 2023

Area of Science:

  • Biochemistry
  • Radiochemistry
  • Cardiovascular Research

Background:

  • Native low-density lipoprotein (LDL) uptake differs from modified LDL scavenging in atherosclerosis.
  • The impact of LDL preparation and radiolabeling on its structure and imaging is not well understood.

Purpose of the Study:

  • To investigate whether 99mTc-labeled LDL represents native or modified LDL.
  • To evaluate the influence of different 99mTc labeling methods on LDL oxidation.

Main Methods:

  • Compared three 99mTc labeling methods (dithionite, borohydride, ascorbic acid).
  • Assessed oxidation-relevant parameters: lag-time, TBARS, REM, baseline dienes, lipid peroxides, amino-groups, and thiol-groups.
  • Expressed results as percentage of freshly isolated native LDL or control LDL.

Main Results:

  • All three labeling methods induced some degree of LDL oxidative modification.
  • Baseline dienes were most affected by borohydride and ascorbic acid methods.
  • Lag-time increased with borohydride but decreased with ascorbic acid; no change with dithionite.
  • TBARS levels increased with dithionite and borohydride, but decreased with ascorbic acid.
  • Thiol groups increased with dithionite, slightly with ascorbic acid, and decreased with borohydride.
  • Lipid peroxide generation increased only with the borohydride method.
  • Relative electrophoretic mobility (REM) increased with dithionite and borohydride methods.

Conclusions:

  • All tested 99mTc labeling methods result in oxidatively modified LDL.
  • Imaging native LDL using 99mTc-labeled LDL is not feasible.
  • The ascorbic acid method demonstrates antioxidant properties and offers some protection against LDL modification.