Related Experiment Video
Updated: Jul 19, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity
Published on: June 12, 2016
Application of an improved column characterisation system to evaluate the within and between batch variability
Erik Haghedooren1, Agnes Kerner, Béla Noszál
1Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Analyse, O&N 2, PB 923, Herestraat 49, B-3000 Leuven, Belgium.
Abstract:
The selection of a reversed-phase liquid chromatographic column with suitable selectivity for a particular separation is difficult if the brand name of the column is not known. A project to develop a chromatographic test procedure to characterize reversed-phase liquid chromatography C18 columns was started earlier and resulted in a fast, simple, repeatable and reproducible test procedure using four column parameters. Here, this procedure is used to evaluate the diversity of columns originating from the same batch as well as from different batches. The determination of one of the parameters, the retention factor of 2,2'-dipyridyl, was improved and a simplified test procedure is proposed.
Related Concept Videos
Column Efficiency: Rate Theory
During elution, a solute molecule experiences numerous transitions between stationary and mobile phases, exhibiting irregular residence times in...
Testing a Claim about Standard Deviation
The hypothesis testing for the claim of population standard deviation (or variance) requires the data and samples to be random and unbiased. The population distribution also must be normal. There is no specific requirement on the sample size as the estimation is based on the chi-square distribution.
As a first step, the hypothesis (null and alternative) concerning the claim about...
Principles Of Column Chromatography
Column Efficiency: Plate Theory
A higher number of theoretical plates signifies better column efficiency and improved separation capabilities. Plate height affects bandwidth and separation quality; it is inversely...
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Data Validation
Key parameters for method validation include:

