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Atrial peptides induce mast cell histamine release.
T J Opgenorth1, G P Budzik, K W Mollison
1Abbott Laboratories, Abbott Park, IL 60064.
Peptides
|September 1, 1990
Summary
Human atrial natriuretic peptides, positively charged molecules, trigger histamine release from mast cells, increasing skin permeability. This suggests a role in allergic reactions and inflammation.
Area of Science:
- Biochemistry
- Immunology
- Physiology
Background:
- Human atrial natriuretic peptide [ANF(1-28)] is a positively charged peptide.
- Mast cells are key players in allergic and inflammatory responses.
- Previous research suggests positively charged peptides can induce mast cell degranulation.
Purpose of the Study:
- To investigate the potential of atrial peptides to induce histamine release from mast cells.
- To examine the in vivo effects of atrial peptides on skin permeability.
Main Methods:
- In vitro: Isolated rat peritoneal mast cells were exposed to different atrial peptides (ANF(1-28), ANF(3-28), ANF(5-28)).
- Histamine release was measured in a dose-dependent manner.
- In vivo: Rat skin permeability was assessed after administration of ANF(3-28).
- The effect of histamine and serotonin antagonists on skin permeability was evaluated.
Main Results:
- Atrial peptides [ANF(1-28), (3-28), and (5-28)] induced dose-dependent histamine release from isolated rat mast cells.
- ANF(3-28) caused a dose-dependent increase in rat skin permeability in vivo.
- This increase in skin permeability was significantly reduced by histamine and serotonin antagonists.
Conclusions:
- Atrial peptides are capable of inducing mast cell degranulation.
- The findings suggest atrial peptides may contribute to inflammatory processes by releasing histamine.
- These peptides act similarly to other positively charged peptides in mast cell activation.