Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A microscopic electrostatic model for the amphotericin B channel.

M Bonilla-Marín1, M Moreno-Bello, I Ortega-Blake

  • 1Depto. de Física, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City.

Biochimica Et Biophysica Acta
|January 9, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-Phase Solvation Model for Biological Membranes: Molecular Action Mechanism of Amphotericin B.

Journal of chemical theory and computation·2017
Same author

Myo1g is an active player in maintaining cell stiffness in B-lymphocytes.

Cytoskeleton (Hoboken, N.J.)·2016
Same author

Theoretical study of the aqueous solvation of HgCl2: Monte Carlo simulations using second-order Moller-Plesset-derived flexible polarizable interaction potentials.

The Journal of chemical physics·2012
Same author

Aqueous solvation of As(OH)3: a Monte Carlo study with flexible polarizable classical interaction potentials.

The Journal of chemical physics·2010
Same author

Effect of membrane structure on the action of polyenes II: nystatin activity along the phase diagram of ergosterol- and cholesterol-containing POPC membranes.

The Journal of membrane biology·2010
Same author

Effect of membrane structure on the action of polyenes: I. Nystatin action in cholesterol- and ergosterol-containing membranes.

The Journal of membrane biology·2010

This study proposes a microscopic model for amphotericin B channels, explaining ion permeability and selectivity. The model supports the idea that these channels are formed by a variable number of antibiotic molecules.

Area of Science:

  • Biophysics
  • Computational Chemistry
  • Pharmacology

Background:

  • Amphotericin B forms pores in cell membranes, crucial for its antifungal activity.
  • The exact mechanism of ion transport through these pores remains incompletely understood.

Purpose of the Study:

  • To develop a microscopic model of amphotericin B channels.
  • To explain the observed ion permeability and selectivity of these channels.

Main Methods:

  • Utilized atomic coordinates from X-ray diffraction for pore structure.
  • Calculated electrostatic energy profiles using Mulliken charges for monovalent ion passage.
  • Modeled water as a continuum and included membrane effects.

Main Results:

Related Experiment Videos

  • The model successfully explains amphotericin B channel permeability and selectivity.
  • Electrostatic profiles support the hypothesis of variable unit channels.
  • Provided insights into unexplained experimental observations.

    • Conclusions:

    • The proposed microscopic model accurately represents amphotericin B channels.
    • Channel formation by a variable number of amphotericin B molecules is supported.
    • The model offers a framework for understanding antifungal drug mechanisms.