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Updated: Mar 22, 2026

Traction Force Microscopy to Study B Lymphocyte Activation
Published on: July 23, 2020
Myo1g is an active player in maintaining cell stiffness in B-lymphocytes
O López-Ortega1,2, E Ovalle-García3, I Ortega-Blake4
1Departamento De Biomedicina Molecular, Centro De Investigación Y De Estudios Avanzados Del Instituto Politécnico Nacional, Ciudad De México, C. P. 07360, México.
Abstract:
B-lymphocytes are migrating cells that specialize in antigen presentation, antibody secretion, and endocytosis; these processes implicate the modulation of plasma membrane elasticity. Cell stiffness is a force generated by the interaction between the actin-cytoskeleton and the plasma membrane, which requires the participation of several proteins. These proteins include class I myosins, which are now considered to play a role in controlling membrane-cytoskeleton interactions. In this study, we identified the motor protein Myosin 1g (Myo1g) as a mediator of this phenomenon. The absence of Myo1g decreased the cell stiffness, affecting cell adhesion, cell spreading, phagocytosis, and endocytosis in B-lymphocytes. The results described here reveal a novel molecular mechanism by which Myo1g mediates and regulates cell stiffness in B-lymphocytes. © 2016 Wiley Periodicals, Inc.
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