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[Study on determination of entrapment efficiency of sinomenine liposomes]
Li-ling Zhou1, Yan Wang, Qing-fei Liu
1Guangzhou University of Traditional Chinese Medicine, Guangzhou, China. zhouliling717@sina.com
Objective:
To establish an HPLC method for the determination of entrapment efficiency of sinomenine liposomes.
Method:
The liposomes and dissociated drugs were separated by sephadex filtration, mini-column centrifugation and dialysis. The methodology study and the optimization of determining condition were carried out at the same time.
Result:
Sephadex filtration could effectively separate the sinomenine liposomes from dissociated sinomenine. The column recovery was 98.8%, the average entrapment efficiency of three tests was64.9%, RSD 2.67%.
Conclusion:
The method was simple, exact, and had a good reappearance. It can be used to examine the entrapment efficiency of sinomenine liposomes.
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