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Anti-inflammatory effects of a novel peptide designed to bind with NF-kappaB p50 subunit
Zhen-ping Wang1, Shao-xi Cai, Dong-bo Liu
1Bioengineering College, Chongqing University, Chongqing 400044, China.
Aim:
To explore the anti-inflammatory effects of a novel peptide designed to bind with the NF-kappaB p50 subunit.
Methods:
The affinity of the peptide binding with p50 was measured with a biosensor. Levels of tumor necrosis factor-alpha(TNF-alpha) and interleukin 6 (IL-6) from a human acute monocytic leukemia cell line (THP-1) treated with lipopolysaccharide (LPS) were measured using the ELISA method. In vivo anti-inflammatory effects of the peptide were tested with phorbol myristate acetate (PMA)-induced ear edema and zymosan A-induced peritonitis in mice.
Results:
The peptide has the ability to interact with the NF-kappaB p50 subunit and can effectively inhibit TNF-alpha and IL-6 production in the THP-1 cell line, PMA-induced ear edema and zymosan A-induced peritonitis in mice.
Conclusion:
The peptide may have therapeutic potential for the treatment of local acute inflammation.
