Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

SPIRE: the SPIDER reconstruction engine.

William T Baxter1, ArDean Leith, Joachim Frank

  • 1Wadsworth Center, Empire State Plaza, Albany, NY 12201-0509, USA.

Journal of Structural Biology
|October 24, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural studies of nedicistrovirus IRES-driven, initiation factor-independent translation shed light on key steps of eukaryotic translation elongation.

Nucleic acids research·2026
Same author

The mechanism of ribosomal recruitment during translation initiation on the Type 2 encephalomyocarditis virus IRES.

The EMBO journal·2026
Same author

Structures of naked mole-rat, tuco-tuco, and guinea pig ribosomes-is rRNA fragmentation linked to translational fidelity?

Nucleic acids research·2026
Same author

Calculation of specificity constants Γ for RUBISCO of different species from microcalorimetric data.

Photosynthesis research·2025
Same author

Structural Studies of Nedicistrovirus IRES-Driven, Initiation Factor-independent Translation Shed Light on Key Steps of Eukaryotic Translation Elongation.

bioRxiv : the preprint server for biology·2025
Same author

Structures of Naked Mole-Rat, Tuco-Tuco, and Guinea Pig Ribosomes-Is rRNA Fragmentation Linked to Translational Fidelity?

bioRxiv : the preprint server for biology·2025
Same journal

Ultrastructural evidence of autophagy-related processes and mitochondrial remodeling in the myxozoan parasite Henneguya piaractus.

Journal of structural biology·2026
Same journal

Architecture and dynamics of a supramolecular oxygen transport system in human homogentisate 1,2-Dioxygenase.

Journal of structural biology·2026
Same journal

Connecting pathways between mineralized fibrocartilage and bone at the Achilles tendon insertion.

Journal of structural biology·2026
Same journal

Structural and functional characterization of thermostable EstS1 esterase for BHET degradation.

Journal of structural biology·2026
Same journal

Following the white rabbit: multiscale 2D3D correlative imaging of bone structure.

Journal of structural biology·2026
Same journal

The mantis shrimp eye imaged in 3D using 4th generation synchrotron multiscale phase contrast tomography.

Journal of structural biology·2026
See all related articles

SPIRE is a new Python program that modernizes user interaction with the SPIDER image processing system for electron microscopy. It offers a graphical user interface and database connectivity for streamlined reconstruction projects.

Area of Science:

  • Microscopy
  • Image Processing
  • Computational Biology

Background:

  • Electron microscopy requires complex image processing for reconstruction.
  • Existing systems like SPIDER can have user interaction challenges.
  • Modernizing scientific software enhances research efficiency.

Purpose of the Study:

  • To develop a user-friendly interface for the SPIDER image processing system.
  • To streamline electron microscopical reconstruction workflows.
  • To improve data management and parameter handling in reconstruction projects.

Main Methods:

  • Developed SPIRE, a Python program with a graphical user interface (GUI).
  • Integrated SPIRE with the SPIDER system for batch file execution.
  • Implemented XML-tagged configuration files for project flexibility.

Related Experiment Videos

  • Enabled database connectivity for parameter download and result upload.

    • Main Results:

    • SPIRE provides an intuitive GUI for SPIDER command execution.
    • Users can easily monitor project status and generated data.
    • Configuration files allow customization for different reconstruction projects.
    • Seamless integration with laboratory databases for parameter and result management.

    Conclusions:

    • SPIRE successfully modernizes user interaction with SPIDER.
    • The software enhances the efficiency and accessibility of electron microscopy reconstruction.
    • SPIRE's flexible design supports diverse research needs in structural biology.