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Measurement of hydrogen peroxide in an advanced oxidation process using an automated biosensor
B Modrzejewska1, A J Guwy, R Dinsdale
1School of Applied Sciences, University of Glamorgan, Pontypridd, Mid Glamorgan CF37 1DL, UK.
Water Research
|October 24, 2006
Summary
A novel gas-phase biosensor effectively monitors hydrogen peroxide in advanced oxidation processes. This technology enhances process control and efficiency by providing real-time measurements resistant to industrial fouling.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Biotechnology
Background:
- Advanced Oxidation Processes (AOPs) are crucial for industrial wastewater treatment.
- Accurate monitoring of hydrogen peroxide (H2O2) is essential for optimizing AOP efficiency.
- Existing monitoring methods can suffer from fouling and enzyme degradation in industrial settings.
Purpose of the Study:
- To develop and evaluate a novel biosensor for real-time hydrogen peroxide monitoring in AOPs.
- To assess the biosensor's resistance to fouling and enzyme stability.
- To compare the biosensor's performance against traditional UV spectroscopy methods.
Main Methods:
- A gas-phase hydrogen peroxide biosensor utilizing continuously supplied catalase was employed.
- The biosensor was integrated into a UV/hydrogen peroxide immobilized Fenton AOP.
- Interference from dissolved gases was mitigated using nitrogen sparging.
Main Results:
- The biosensor demonstrated a linear response within the 30-400 mg H2O2 L−1 range (R² = 0.99).
- High correlation (0.96–0.99) was observed between the biosensor and UV spectroscopy for residual peroxide determination.
- Nitrogen sparging effectively reduced interference from dissolved gases produced during the AOP.
Conclusions:
- The developed gas-phase biosensor offers a robust and reliable method for monitoring hydrogen peroxide in industrial AOPs.
- Continuous catalase supply ensures enzyme stability and overcomes limitations of immobilized enzyme biosensors.
- This biosensor technology has the potential to significantly improve AOP effectiveness through precise hydrogen peroxide control.
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