Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain
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Updated: Jul 19, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function
Published on: May 14, 2021
Steven L Alam1, Charles Langelier, Frank G Whitby
1Department of Biochemistry, University of Utah, Salt Lake City, Utah 84112-5650, USA.
Abstract:
The ESCRT-I and ESCRT-II complexes help sort ubiquitinated proteins into vesicles that accumulate within multivesicular bodies (MVBs). Crystallographic and biochemical analyses reveal that the GLUE domain of the human ESCRT-II EAP45 (also called VPS36) subunit is a split pleckstrin-homology domain that binds ubiquitin along one edge of the beta-sandwich. The structure suggests how human ESCRT-II can couple recognition of ubiquitinated cargoes and endosomal phospholipids during MVB protein sorting.
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