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Published on: February 16, 2022
Simultaneous analysis of nitrate and nitrite in a microfluidic device with a Cu-complex-modified electrode
Muhammad J A Shiddiky1, Mi-Sook Won, Yoon-Bo Shim
1Department of Chemistry and Center for Innovative BioPhysio Sensor Technology, Pusan National University, Busan, South Korea.
Electrophoresis
|October 24, 2006
Summary
A novel capillary electrophoresis microsystem with a modified electrode enables simultaneous nitrite and nitrate detection. This electrocatalytic method offers high sensitivity and reproducibility for analyzing these important analytes in various samples.
Area of Science:
- Analytical Chemistry
- Electrochemistry
- Microsystems Engineering
Background:
- Nitrite and nitrate are crucial analytes in environmental and biological samples.
- Accurate and simultaneous determination of nitrite and nitrate remains a challenge.
- Electrochemical methods offer sensitive detection but often require specific modifications for complex matrices.
Purpose of the Study:
- To develop a sensitive and reproducible method for simultaneous nitrite and nitrate analysis.
- To integrate capillary electrophoresis (CE) with a novel electrochemical sensor.
- To demonstrate the applicability of the developed system for real-world sample analysis.
Main Methods:
- Fabrication of a copper-(3-mercaptopropyl) trimethoxysilane (Cu-MPS) complex-modified carbon paste electrode (CPE).
- Coupling of the modified electrode with a CE microsystem for analyte separation.
- Optimization of CE separation parameters (buffer, pH, field strength) and electrochemical detection potential.
- Characterization of the Cu-MPS complex using voltammetry, XPS, and FT-IR.
Main Results:
- The developed CE-microsystem with Cu-MPS-CPE achieved simultaneous electrocatalytic reduction and detection of nitrite and nitrate.
- Optimized conditions yielded efficient separation within 90 seconds at -250 V/cm.
- The method demonstrated high reproducibility (RSD < 3.2%) with negligible electrode fouling.
- Wide linear dynamic ranges (0.25–120 µM) and low detection limits (0.09 µM for nitrite, 0.08 µM for nitrate) were achieved.
- Successful application in water and urine sample analysis was demonstrated.
Conclusions:
- The Cu-MPS-CPE coupled with CE microsystem provides a robust platform for simultaneous nitrite and nitrate determination.
- The method exhibits excellent sensitivity, reproducibility, and selectivity.
- This approach holds significant potential for routine analysis of nitrite and nitrate in diverse sample types.
